Mass Spectrometry Analysis of Fatty Acid Binding to Albumin
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Albumin is responsible for the transportation of numerous amphipathic molecules including fatty acids in our blood. The number of fatty acids bound to each albumin fluctuates under physiologic and pathologic conditions (2). Current biochemical methods used for the analysis of the interaction requires the use of radioactive isotope (3) and/or enzymatic methods (1) which are unable to specify the type of fatty acids bound to albumin. MALDI-ToF-MS is a powerful tool capable of analysis of small and large biomolecules. Here we examine the potential of MALDI-ToF-MS in analyzing fatty acid binding to albumin.