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dc.contributor.advisorBashur, Christopher
dc.contributor.authorBirthare, Karamveer
dc.creatorBirthare, Karamveer
dc.date.accessioned2015-06-10T20:27:58Z
dc.date.available2015-06-10T20:27:58Z
dc.date.issued2015-05
dc.identifier.urihttp://hdl.handle.net/11141/679
dc.descriptionThesis (M.S.) - Florida Institute of Technology, 2015en_US
dc.description.abstractCurrent surgical alternatives for treating coronary artery diseases are limited in patients with systemic vascular diseases due to unavailability of suitable autologous vessels. The overall goal of this research project is to develop an appropriate tissue engineered vascular grafts (TEVGs) for small-diameter (<6 mm) arteries. Current grafts fail from intimal hyperplasia, caused by hyperproliferation of the smooth muscle cells (SMCs), and graft thrombosis, caused by lack of functional endothelium. Formation of the endothelium, and production of pro-inflammatory species that limits the long term patency, are partially due to the accumulation of oxidized lipid species. In this study, the scaffold composition was systematically varied to assess the inflammatory response and lipid oxidation levels in a rat peritoneal model. Specifically, we determined the collagen to PCL ratio required to limit the production of pro-inflammatory species, while maintaining the required mechanical strength for vascular grafts. Electrospun conduits were prepared from 0%, 10%, and 25% blends of collagen/ PCL (w/w) and implanted in the rat peritoneal cavity for 4 weeks. The results of the study showed that scaffold composition can be varied while keeping the fiber diameter similar. Results from 10% blend conduits showed significantly higher expression for contractile markers compared to the 25% and 0% blend conditions. In general, adding collagen to the PCL conduits reduced the accumulation of oxidized species within the implanted conduits. All conduits exhibited sufficient tensile strength post- implantation. In conclusion, these results demonstrate that introducing 10% collagen into electrospun scaffolds limits the pro-inflammatory characteristics of the recruited peritoneal cells that can potentially improve patency of the TEVGs.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.rightsCC BY Creative Commons with Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en_US
dc.titleImpact of Collagen-Incorporation within Electrospun Vascular Conduits on the Inflammatory Response and Conduit Mechanicsen_US
dc.typeThesisen_US
dc.date.updated2015-05-05T12:31:06Z
thesis.degree.nameMaster of Science in Biomedical Engineeringen_US
thesis.degree.levelMastersen_US
thesis.degree.disciplineBiomedical Engineeringen_US
thesis.degree.departmentBiomedical Engineeringen_US
thesis.degree.grantorFlorida Institute of Technologyen_US
dc.type.materialtext


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