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dc.contributor.authorHelmstetter, Charles E.
dc.date.accessioned2015-05-26T13:36:55Z
dc.date.available2015-05-26T13:36:55Z
dc.date.issued2015-03-25
dc.identifier.citationHelmstetter, C. E. A ten-year search for synchronous cells: obstacles, solutions, and practical applications. Frontiers in Microbiology, 6 (2015)en_US
dc.identifier.urihttp://hdl.handle.net/11141/673
dc.description.abstractMy effort to use synchronously dividing cultures to examine the Escherichia coli cell cycle involved a 10-year struggle with failure after failure punctuated by a few gratifying successes, especially at the end. In this essay, I recount my personal journey in this obsessive experimental pursuit. That narrative is followed by a description of a simplified version of the "baby machine," a technique that was developed to obtain minimally disturbed, synchronously growing E. coli cells. Subsequent studies with this methodology led to an understanding of the basic properties of the relationship between chromosome replication and cell division. Accordingly, I end this reminiscence with a simple, fool-proof graphical strategy for deducing the pattern of chromosome replication during the division cycle of cells growing at any rate.en_US
dc.language.isoen_USen_US
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleA ten-year search for synchronous cells: obstacles, solutions, and practical applicationsen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2015.00238


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This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.