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dc.contributor.authorStricker, Stephen A.
dc.contributor.authorCarroll, David J.
dc.contributor.authorTsui, Wai L.
dc.date.accessioned2017-12-08T01:01:44Z
dc.date.available2017-12-08T01:01:44Z
dc.date.issued2010
dc.identifier.citationStricker, S.A., Carroll, D.J., Tsui, W.L. Roles of Src family kinase signaling during fertilization and the first cell cycle in the marine protostome worm Cerebratulus (2010) International Journal of Developmental Biology, 54 (5), pp. 787-793. Cited 15 times.en_US
dc.identifier.urihttp://hdl.handle.net/11141/2224
dc.descriptionEgg activation, Nemertean, PLC, PP2, U73122en_US
dc.description.abstractFor eggs to generate a calcium response during fertilization, the sperm of many deuterostome animals must first activate a group of egg kinases, called Src family kinases (SFKs). However, whether SFK activation is also required for fertilization-induced calcium signals in eggs of protostomes remains unknown. Thus, in this study, unfertilized oocytes of the marine protostome worm Cerebratulus were treated with either PP2 to inhibit SFKs or with U73122 to block phospholipase C activity downstream of SFK. Compared with control fertilizations, the inhibitors significantly reduced post-insemination levels of polar body formation and cleavage, but apparently did so via different mechanisms, based on the variable effects of these drugs on sperm incorporations and pronuclear differentiation. Moreover, confocal calcium imaging revealed that repetitive calcium waves (=oscillations) were blocked by U73122, but not by PP2, even though immunoblots indicated SFK activity was inhibited by PP2. Such findings fail to support the view that SFKs are required for initiating fertilization-induced calcium oscillations in Cerebratulus, and alternative mechanisms for the observed inhibition of polar body formation and cleavage in drug-treated specimens are discussed. © 2010 UBC Press.en_US
dc.language.isoen_USen_US
dc.rights© 2010 UBC Pressen_US
dc.rights.urihttp://www.ijdb.ehu.es/web/descarga/instructionsen_US
dc.titleRoles of Src family kinase signaling during fertilization and the first cell cycle in the marine protostome worm Cerebratulusen_US
dc.typeArticleen_US
dc.identifier.doi10.1387/ijdb.092918ss


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