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dc.contributor.authorArthaut, Louis David
dc.contributor.authorJourdan, Nathalie
dc.contributor.authorMteyrek, Ali
dc.contributor.authorProcopio, Maria
dc.contributor.authorEl-Esawi, Mohamed A.
dc.contributor.authorD'Harlingue, Alain
dc.contributor.authorBouchet, Pierre Etienne
dc.contributor.authorWitczak, Jacques
dc.contributor.authorRitz, Thorsten
dc.contributor.authorMartino, Carlos F.
dc.date.accessioned2017-10-12T18:47:22Z
dc.date.available2017-10-12T18:47:22Z
dc.date.issued2017-03-15
dc.identifier.citationArthaut, L.-D., Jourdan, N., Mteyrek, A., Procopio, M., El-Esawi, M., D'Harlingue, A., Bouchet, P.-E., Witczak, J., Ritz, T., Klarsfeld, A., Birman, S., Usselman, R.J., Hoecker, U., Martino, C.F., Ahmad, M. Blue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycle (2017) PLoS ONE, 12 (3), art. no. e0171836, .en_US
dc.identifier.urihttp://hdl.handle.net/11141/1883
dc.descriptioncryptochrome, reactive oxygen metaboliteen_US
dc.description.abstractCryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila melanogaster cryptochrome (DmCry) absorbs light through a flavin (FAD) cofactor that undergoes photoreduction to the anionic radical (FAD) redox state both in vitro and in vivo. However, recent efforts to link this photoconversion to the initiation of a biological response have remained controversial. Here, we show by kinetic modeling of the DmCry photocycle that the fluence dependence, quantum yield, and half-life of flavin redox state interconversion are consistent with the anionic radical (FAD∗-) as the signaling state in vivo. We show by fluorescence detection techniques that illumination of purified DmCry results in enzymatic conversion of molecular oxygen (O2) to reactive oxygen species (ROS). We extend these observations in living cells to demonstrate transient formation of superoxide (O2 ∗-), and accumulation of hydrogen peroxide (H2O2) in the nucleus of insect cell cultures upon DmCry illumination. These results define the kinetic parameters of the Drosophila cryptochrome photocycle and support light-driven electron transfer to the flavin in DmCry signaling. They furthermore raise the intriguing possibility that light-dependent formation of ROS as a byproduct of the cryptochrome photocycle may contribute to its signaling role. © 2017 Arthaut et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.language.isoen_USen_US
dc.rights© 2017 the Authors. Licensed under Creative Commons Attribution Licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleBlue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycleen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0171836


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© 2017 the Authors.  Licensed under Creative Commons Attribution License
Except where otherwise noted, this item's license is described as © 2017 the Authors. Licensed under Creative Commons Attribution License