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dc.contributor.authorSaxena, Rahul K.
dc.contributor.authorVasudevan, Sona
dc.contributor.authorPatil, Digvijay
dc.contributor.authorAshoura, Norah
dc.contributor.authorGrimwade, Julia E.
dc.contributor.authorCrooke, Elliott
dc.date.accessioned2017-10-06T17:34:48Z
dc.date.available2017-10-06T17:34:48Z
dc.date.issued2015-11-24
dc.identifier.citationSaxena, R., Vasudevan, S., Patil, D., Ashoura, N., Grimwade, J.E., Crooke, E. Nucleotide-induced conformational changes in Escherichia coli DnaA protein are required for bacterial ORC to Pre-RC conversion at the chromosomal origin (2015) International Journal of Molecular Sciences, 16 (11), pp. 27897-27911. Cited 2 times.en_US
dc.identifier.urihttp://hdl.handle.net/11141/1811
dc.descriptionAAA+ domain, Chromosomal origin, DNA replication, DnaA protein, Molecular docking, Proteolysisen_US
dc.description.abstractDnaA oligomerizes when bound to origins of chromosomal replication. Structural analysis of a truncated form of DnaA from Aquifex aeolicus has provided insight into crucial conformational differences within the AAA+ domain that are specific to the ATP- versus ADP- bound form of DnaA. In this study molecular docking of ATP and ADP onto Escherichia coli DnaA, modeled on the crystal structure of Aquifex aeolicus DnaA, reveals changes in the orientation of amino acid residues within or near the vicinity of the nucleotide-binding pocket. Upon limited proteolysis with trypsin or chymotrypsin ADP-DnaA, but not ATP-DnaA generated relatively stable proteolytic fragments of various sizes. Examined sites of limited protease susceptibility that differ between ATP-DnaA and ADP-DnaA largely reside in the amino terminal half of DnaA. The concentration of adenine nucleotide needed to induce conformational changes, as detected by these protease susceptibilities of DnaA, coincides with the conversion of an inactive bacterial origin recognition complex (bORC) to a replication efficient pre-replication complex (pre-RC) at the E. coli chromosomal origin of replication (oriC). © 2015 by the authors; licensee MDPI, Basel, Switzerland.en_US
dc.language.isoen_USen_US
dc.rights©2015 the authorsen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleNucleotide-induced conformational changes in Escherichia coli DnaA protein are required for bacterial ORC to Pre-RC conversion at the chromosomal originen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms161126064


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